You can follow the same steps to enable it again. Click on Yes, and now your mic will not function. Right-click on Microphone and select Disable.Ī dialog box will prompt with a warning. In the Device Manager window, expand the Audio inputs and outputs section and you will see your Microphone listed there as one of the interfaces. Right-click the Start Button and open Device Manager. There are a few several ways to disable the microphone in Windows 10 from different settings locations.